3SYX
Crystal Structure of the WH1 domain from human sprouty-related, EVH1 domain-containing protein. Northeast Structural Genomics Consortium Target HR5538B.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97896 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 52.754, 52.754, 121.638 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.377 - 2.453 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xod |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.664 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.057 | 0.691 |
| Number of reflections | 27739 | |
| <I/σ(I)> | 29.6 | 2.5 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch crystallization under oil | 6 | 277 | 12% PEG 20000, 0.1 M potassium acetate, 10 mM yttrium(III) chloride, 0.1 M MES, pH 6.0, Microbatch crystallization under oil, temperature 277K |
