3SYO
Crystal structure of the G protein-gated inward rectifier K+ channel GIRK2 (Kir3.2) in complex with sodium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 85.737, 85.737, 178.602 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.680 - 3.540 |
R-factor | 0.2613 |
Rwork | 0.260 |
R-free | 0.27270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e4f |
RMSD bond length | 0.008 |
RMSD bond angle | 1.118 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.685 | 41.685 | 3.700 |
High resolution limit [Å] | 3.540 | 7.680 | 3.570 |
Rmerge | 0.158 | 0.058 | 0.729 |
Number of reflections | 7975 | ||
<I/σ(I)> | 6.4 | ||
Completeness [%] | 92.0 | 90.2 | 76.8 |
Redundancy | 8.1 | 7.9 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.15 | 50 mM sodium citrate, pH 6.0, 1 M sodium chloride, 30-35% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |