3SYB

Crystal structure of Pseudomonas aeruginosa OccD3 (OpdP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-17
Detector	ADSC QUANTUM 270
Wavelength(s)	0.972
Spacegroup name	C 2 2 21
Unit cell lengths	99.930, 159.828, 82.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.993 - 2.700
R-factor	0.2194
Rwork	0.214
R-free	0.26710
Structure solution method	P
RMSD bond length	0.008
RMSD bond angle	1.163
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.710
High resolution limit [Å]	2.660	2.660
Number of reflections	18213
Completeness [%]	92.9	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	295	25% PEG 1000 0.2 M ammonium sulphate 50 mM Na-citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K