3SWM

The NAC domain of ANAC019 in complex with DNA, gold derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.038
Spacegroup name	P 21 21 21
Unit cell lengths	68.530, 109.070, 173.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.203 - 4.250
R-factor	0.2562
Rwork	0.250
R-free	0.30920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1UT7 + B-DNA model
RMSD bond length	0.015
RMSD bond angle	2.285
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.203	4.450
High resolution limit [Å]	4.250	4.250
Number of reflections	9600
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.01 M magnesium sulfate, 0.05 M MES, pH 6.5, 0.5% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298K