3SW9
GLP (G9a-like protein) SET domain in complex with Dnmt3aK44me0 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07169 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.140, 91.140, 95.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.689 - 3.050 |
| R-factor | 0.1765 |
| Rwork | 0.173 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mo5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.222 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.690 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.120 | 0.445 |
| Number of reflections | 13788 | |
| <I/σ(I)> | 13 | 3.9 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 6.2 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M Hepes, 16% polyethylene glycol 4000 and 8% isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
