3STB

A complex of two editosome proteins and two nanobodies

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	77
Detector technology	PIXEL
Collection date	2010-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792, 0.9794, 0.9116
Spacegroup name	P 43 21 2
Unit cell lengths	74.540, 74.540, 239.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.530 - 2.500
R-factor	0.20069
Rwork	0.199
R-free	0.23400
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.328
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.530	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.125	0.361
<I/σ(I)>	17.49	2.333
Completeness [%]	97.1	77.3
Redundancy		5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES pH 7.5, 10% 2-propanol, 22% w/v PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES pH 7.5, 10% 2-propanol, 22% w/v PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K