3ST0
Engineered medium-affinity halide-binding protein derived from YFP: halide-free
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1109 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.026, 62.964, 69.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.534 - 1.190 |
| R-factor | 0.1419 |
| Rwork | 0.141 |
| R-free | 0.16600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2SVC |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.292 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.534 | 50.000 | 1.210 |
| High resolution limit [Å] | 1.190 | 3.230 | 1.190 |
| Rmerge | 0.044 | 0.031 | 0.432 |
| Number of reflections | 66845 | ||
| <I/σ(I)> | 19.9 | ||
| Completeness [%] | 92.7 | 85.5 | 55.5 |
| Redundancy | 7.4 | 6.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 290 | 10% PEG3000, 150 mM ammonium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
