3SS4

Crystal structure of mouse Glutaminase C, phosphate-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-08
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	97.720, 136.280, 176.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.908 - 2.850
R-factor	0.2291
Rwork	0.227
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3czd
RMSD bond length	0.013
RMSD bond angle	1.021
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.500	3.000
High resolution limit [Å]	2.850	2.850
Rmerge	0.152
Number of reflections	51943
<I/σ(I)>	5.1	1.7
Completeness [%]	94.2	91
Redundancy	3.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	17% PEG3350, 0.2M NaCl, 0.1M Bis-TRIS, VAPOR DIFFUSION, SITTING DROP, temperature 291K, pH 6.5