3SQO
PCSK9 J16 Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.921, 70.981, 109.156 |
| Unit cell angles | 90.00, 95.90, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.2261 |
| Rwork | 0.223 |
| R-free | 0.28280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.062 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 5.700 | 2.650 |
| Rmerge | 0.119 | 0.048 | 0.472 |
| Number of reflections | 35342 | ||
| <I/σ(I)> | 10.2 | 15.3 | |
| Completeness [%] | 94.7 | 98.1 | 73.5 |
| Redundancy | 2.9 | 3.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 7% PEG 10000, 100 mM Hepes pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
