3SQI
DNA binding domain of Ndc10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-06-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97914 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 117.303, 147.536, 95.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.388 - 2.825 |
| R-factor | 0.1962 |
| Rwork | 0.193 |
| R-free | 0.25110 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.113 | 0.717 |
| Number of reflections | 18620 | |
| <I/σ(I)> | 23.8 | 2 |
| Completeness [%] | 91.3 | 74.2 |
| Redundancy | 14.7 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 20% PEG400, 0.1 M MES pH 6.0, 0.2 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
