3SPW
Structure of Osh4p/Kes1p in complex with phosphatidylinositol 4-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.460, 54.760, 121.860 |
| Unit cell angles | 90.00, 91.00, 90.00 |
Refinement procedure
| Resolution | 47.290 - 2.600 |
| R-factor | 0.2302 |
| Rwork | 0.228 |
| R-free | 0.26960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zhw |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.596 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.290 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 30180 | |
| <I/σ(I)> | 6.82 | 2.78 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 250 mM CaCl2, 100 mM MES, 16% PEG 20K, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
