3SP3
Lysozyme in 20% sucrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-08-22 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 76.847, 76.847, 37.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.410 - 1.801 |
R-factor | 0.1868 |
Rwork | 0.185 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rnx |
RMSD bond length | 0.008 |
RMSD bond angle | 1.184 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.820 |
High resolution limit [Å] | 1.800 | 1.790 |
Rmerge | 0.061 | 0.391 |
Number of reflections | 11095 | |
<I/σ(I)> | 33.9 | 2.09 |
Completeness [%] | 92.9 | 33.1 |
Redundancy | 6.5 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 277 | 40 mM Sodium Acetate, 150mM Sodium Chloride, 20% Sucrose, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |