3SOK
Dichelobacter nodosus pilin FimA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 105.729, 105.729, 73.848 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.283 - 2.300 |
| R-factor | 0.2393 |
| Rwork | 0.234 |
| R-free | 0.29020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 2HI2 conserved core (residues 1-55 + 78-92 + 105-111) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.098 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.520 |
| High resolution limit [Å] | 2.000 | 2.300 |
| Number of reflections | 27380 | |
| Completeness [%] | 96.5 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 295 | 60% saturated ammonium sulfate 5% saturated NaCl 100mM Na Citrate 5% 1M NaPO4, pH 9.23, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
