3SNK

Crystal structure of a Response regulator CheY-like protein (mll6475) from MESORHIZOBIUM LOTI at 2.02 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-06-21
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 32 2 1
Unit cell lengths	64.859, 64.859, 69.982
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.805 - 2.020
R-factor	0.1988
Rwork	0.198
R-free	0.22210
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.805		2.090
High resolution limit [Å]	2.020	4.340	2.020
Rmerge	0.075	0.056	0.969
Number of reflections	11550	2186	2063
<I/σ(I)>	18.05	39.2	2.7
Completeness [%]	99.7	99.9	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2.0% polyethylene glycol 400, 2.0M ammonium sulfate, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K