3SKW
Crystal structure of the 2'- Deoxyguanosine riboswitch bound to 2'- Deoxyguanosine, cesium soak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.376, 35.414, 119.269 |
| Unit cell angles | 90.00, 95.18, 90.00 |
Refinement procedure
| Resolution | 19.646 - 2.950 |
| R-factor | 0.2242 |
| Rwork | 0.217 |
| R-free | 0.28730 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.045 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.108 | 0.335 |
| Number of reflections | 8847 | |
| <I/σ(I)> | 19.6 | 4.7 |
| Completeness [%] | 95.7 | 98.4 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.05 M Na-HEPES, pH 7.5, ~40 % (v/v) pentaerythritol propoxylate, 0.2 M KCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
