3SK7

Crystal Structure of V. cholerae SeqA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-30
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	29.008, 55.029, 135.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.918 - 1.500
R-factor	0.1587
Rwork	0.156
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fmt
RMSD bond length	0.010
RMSD bond angle	1.068
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.490
High resolution limit [Å]	1.460	1.460
Rmerge	0.082	0.462
Number of reflections	35671
<I/σ(I)>	21.266	4.403
Completeness [%]	99.6	98.3
Redundancy	6.9	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP		277	30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K