3SJQ
Crystal structure of a small conductance potassium channel splice variant complexed with calcium-calmodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.740, 65.630, 66.080 |
| Unit cell angles | 90.68, 110.45, 111.02 |
Refinement procedure
| Resolution | 19.994 - 1.900 |
| R-factor | 0.1711 |
| Rwork | 0.170 |
| R-free | 0.20050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g4y |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 53499 | |
| Completeness [%] | 97.9 | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.65 | 277 | 0.75 M Li2SO4, 0.5 M (NH4)2SO4, 0.1 M Sodium citrate pH 4.65 and Silver Bullets 22 reagent 70, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
