3SI7
The crystal structure of the NBD1 domain of the mouse CFTR protein, deltaF508 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97156 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 170.546, 170.546, 109.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.900 - 2.250 |
| R-factor | 0.1838 |
| Rwork | 0.181 |
| R-free | 0.22740 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.076 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.7_650) |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 76676 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.5 M Sodium Acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
