3SHX
Frog M-ferritin with magnesium, L134P mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.775, 183.775, 183.775 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.380 - 1.350 |
| R-factor | 0.13881 |
| Rwork | 0.137 |
| R-free | 0.16299 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.468 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.071 | 0.807 |
| Number of reflections | 59574 | |
| <I/σ(I)> | 19.5 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 2M MGCL2, 0.1M BICINE, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
