3SH3
Crystal structure of a pro-inflammatory lectin from the seeds of Dioclea wilsonii STANDL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-02 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.47 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 58.820, 66.873, 107.345 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.431 - 2.300 |
R-factor | 0.2162 |
Rwork | 0.214 |
R-free | 0.26150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.155 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.046 |
Number of reflections | 9238 |
Completeness [%] | 93.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.01 M Nickel (II), 0.1 M Tris pH 8.0, 1.0 M lithium sulfate monohydrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |