3SGJ
Unique carbohydrate-carbohydrate interactions are required for high affinity binding between FcgIII and antibodies lacking core fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.654, 88.478, 140.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 140.288 - 2.200 |
| R-factor | 0.19794 |
| Rwork | 0.195 |
| R-free | 0.25173 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.777 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0112) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 140.288 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 45955 | |
| <I/σ(I)> | 14.3 | 1.04 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 0.8264 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 1.4 Sodium Malonate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
