3SGI
Crystal structure of DNA ligase A BRCT domain deleted mutant of Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.437, 99.564, 144.009 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.780 - 3.500 |
| R-factor | 0.31196 |
| Rwork | 0.311 |
| R-free | 0.32210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zau |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.746 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.780 | 3.588 |
| High resolution limit [Å] | 3.500 | 3.497 |
| Number of reflections | 12456 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 89.3 | 94.1 |
| Redundancy | 4.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 12% PEG 8000, 0.1M MES, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
