3SG6
Crystal Structure of Dimeric GCaMP2-LIA(linker 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-02 |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.976, 47.491, 68.673 |
| Unit cell angles | 90.00, 99.72, 90.00 |
Refinement procedure
| Resolution | 27.740 - 1.700 |
| R-factor | 0.20573 |
| Rwork | 0.203 |
| R-free | 0.24942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ek7 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.018 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 40438 | |
| <I/σ(I)> | 15.9 | 1.6 |
| Completeness [%] | 93.9 | 73.9 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, 30% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
