3SFZ

Crystal structure of full-length murine Apaf-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	72.650, 113.070, 243.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.2355
Rwork	0.235
R-free	0.29800
Structure solution method	AB INITIO PHASING
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		3.100
High resolution limit [Å]	3.000	20.000	3.000
Rmerge	0.085	0.043	0.405
Number of reflections	40531	143	3711
<I/σ(I)>	14.26	21.61	3.53
Completeness [%]	98.6	80.8	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1 M HEPES, 1.4 M sodium malonate, 4% v/v gamma-butyrolactone, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K