3SFE
crystal structure of porcine mitochondrial respiratory complex II bound with oxaloacetate and thiabendazole
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.710, 83.420, 294.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.810 |
R-factor | 0.21684 |
Rwork | 0.214 |
R-free | 0.26985 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zoy |
RMSD bond length | 0.007 |
RMSD bond angle | 1.243 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.100 | 0.427 |
Number of reflections | 41586 | |
<I/σ(I)> | 12 | 1.7 |
Completeness [%] | 94.6 | 81.2 |
Redundancy | 4.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 290 | 0.5% n-nonyl-D-maltoside, 1.7mM n-decyl-D-maltoside, 25mM HEPES, 5% PEG 4000, 3% 1,6-hexanediol, 100mM NaCl, 10mM CaCl2, 200mM sucrose, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |