3SEV

Zn-mediated Trimer of Maltose-binding Protein E310H/K314H by Synthetic Symmetrization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	113.780, 115.720, 119.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.060 - 3.050
R-factor	0.2229
Rwork	0.221
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.100
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.060		3.130
High resolution limit [Å]	3.050	13.640	3.050
Rmerge	0.123	0.034	0.543
Number of reflections	30645	379	2231
<I/σ(I)>	10.39	24.86	2.43
Completeness [%]	99.6	91.1	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	0.1M HEPES, 2.4M AMMONIUM SULFATE, pH 8.0, vapor diffusion, hanging drop, temperature 290K