3SEK
Crystal Structure of the Myostatin:Follistatin-like 3 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 82.086, 82.086, 312.691 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.540 - 2.401 |
| R-factor | 0.2469 |
| Rwork | 0.246 |
| R-free | 0.27030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3HH2 (chain B) and 3B4V (chain C) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.012 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.090 | 0.047 | 0.707 |
| Number of reflections | 25257 | ||
| <I/σ(I)> | 12.3 | ||
| Completeness [%] | 99.4 | 98.8 | 95.5 |
| Redundancy | 14.3 | 15.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 293 | potassium thiocyanate, PEG 3350, pH 7.5, hanging drop, temperature 293K |
