3SDF
Crystal Structure of C-lobe of Bovine lactoferrin Complexed with Lipoteichoic acid at 2.1 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 300 |
Detector technology | IMAGE PLATE |
Collection date | 2011-05-18 |
Detector | MARRESEARCH |
Wavelength(s) | 1.541 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.417, 50.398, 65.944 |
Unit cell angles | 90.00, 107.72, 90.00 |
Refinement procedure
Resolution | 62.000 - 2.100 |
R-factor | 0.1991 |
Rwork | 0.197 |
R-free | 0.23441 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rgy |
RMSD bond length | 0.007 |
RMSD bond angle | 1.246 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.820 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 21244 | |
<I/σ(I)> | 12 | 2 |
Completeness [%] | 94.3 | 94.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.01M Znso4, 0.1M MES, 25% PEG, Monomethyl Ether 550 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |