3SC3

Crystal structure of a Putative DNA replication regulator Hda (Sama_1916) from SHEWANELLA AMAZONENSIS SB2B at 3.00 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-15
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	I 2 3
Unit cell lengths	162.440, 162.440, 162.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.881 - 3.001
R-factor	0.2267
Rwork	0.224
R-free	0.25320
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.550
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2007)
Phasing software	SHELX
Refinement software	PHENIX (1.4)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.881		3.170
High resolution limit [Å]	3.000	6.750	3.000
Rmerge	0.143	0.014	0.014
Number of reflections	14422	1324	2175
<I/σ(I)>	10.68	27.2	1.6
Completeness [%]	99.9	99.4	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.26M (NH4)2SO4, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K