3SBR
Pseudomonas stutzeri nitrous oxide reductase, P1 crystal form with substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-04-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 96.577, 106.453, 130.876 |
| Unit cell angles | 111.45, 107.22, 90.72 |
Refinement procedure
| Resolution | 95.000 - 2.240 |
| R-factor | 0.17584 |
| Rwork | 0.173 |
| R-free | 0.22655 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fwx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.317 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 95.050 |
| High resolution limit [Å] | 2.240 |
| Rmerge | 0.090 |
| Number of reflections | 194872 |
| <I/σ(I)> | 12.8 |
| Completeness [%] | 94.6 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 16 % PEG 6000, 0.2 M imidazole/malate buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
