3SB8

Cu-mediated Dimer of T4 Lysozyme D61H/K65H by Synthetic Symmetrization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	44.880, 95.180, 117.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.441 - 2.650
R-factor	0.2247
Rwork	0.222
R-free	0.27280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.739
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.441		2.720
High resolution limit [Å]	2.650	11.850	2.650
Rmerge	0.142	0.051	0.481
Number of reflections	14792	184	1079
<I/σ(I)>	10.34	22.75	3.47
Completeness [%]	97.1	84.8	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	3.5M Sodium Formate, pH 7.0, vapor diffusion, hanging drop, temperature 290K