3SB7

Cu-mediated Trimer of T4 Lysozyme D61H/K65H/R76H/R80H by Synthetic Symmetrization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	H 3
Unit cell lengths	119.910, 119.910, 64.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.472 - 2.700
R-factor	0.1885
Rwork	0.187
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.572
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.472		2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.113	0.049	0.508
Number of reflections	9419	92	685
<I/σ(I)>	9.75	21.33	3.29
Completeness [%]	98.9	86.8	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.2M Potassium Thiocyanate, 25% PEG 3350, vapor diffusion, hanging drop, temperature 290K