3SAH
Crystal structure of the human RRP6 catalytic domain with Y436A mutation in the catalytic site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-18 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 4 |
| Unit cell lengths | 141.000, 141.000, 58.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.990 - 2.650 |
| Rwork | 0.215 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3saf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 5.690 | 2.650 |
| Rmerge | 0.047 | 0.023 | 0.402 |
| Number of reflections | 65066 | ||
| <I/σ(I)> | 15.5 | ||
| Completeness [%] | 99.9 | 99.5 | 99.8 |
| Redundancy | 4.1 | 4.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 6% PEG6000, 1.5 M sodium chloride, 0.4 mM yttrium(III) trichloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
