3S9N
Complex between transferrin receptor 1 and transferrin with iron in the N-Lobe, room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Collection date | 2010-07-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9202 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 234.417, 234.417, 169.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.250 |
| Rwork | 0.254 |
| R-free | 0.28940 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.044 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.370 |
| High resolution limit [Å] | 3.250 | 6.980 | 3.250 |
| Rmerge | 0.218 | 0.172 | 0.985 |
| Number of reflections | 74540 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 99.8 | 98.9 | 100 |
| Redundancy | 8.8 | 8.3 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5% PEG 3350, 0.2 M MgCl2, 10% 1,2-propanediol, pH Hepes 7.5, vapor diffusion, hanging drop, temperature 293K |
