3S8I
The retroviral-like protease (RVP) domain of human DDI1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-05-26 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.981, 41.040, 88.975 |
| Unit cell angles | 90.00, 107.62, 90.00 |
Refinement procedure
| Resolution | 28.270 - 1.700 |
| R-factor | 0.17686 |
| Rwork | 0.176 |
| R-free | 0.19871 |
| Structure solution method | MR |
| Starting model (for MR) | 2ila |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.457 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 30254 | |
| <I/σ(I)> | 52.372 | 7.74 |
| Completeness [%] | 97.8 | 73.4 |
| Redundancy | 6.9 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 21% PEG3350, 0.2 M AMMONIUM PHOSPHATE, 0.1 M BIS-TRIS, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
