3S8A
Structure of Yeast Ribonucleotide Reductase R293A with dGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 108.443, 117.716, 64.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.900 |
| R-factor | 0.23485 |
| Rwork | 0.228 |
| R-free | 0.29965 |
| Structure solution method | Isomorphous |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.643 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.130 | 0.542 |
| Number of reflections | 16450 | |
| <I/σ(I)> | 10.1 | 2.2 |
| Completeness [%] | 96.5 | 98 |
| Redundancy | 4.14 | 4.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 298 | 20-25% PEG 3350, 0.1 M HEPES, 0.1 M sodium chloride, pH 7.0, EVAPORATION, temperature 298K |
