3S7V
Unassembled KI Polyomavirus VP1 Pentamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-02-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.330, 82.780, 142.060 |
| Unit cell angles | 87.01, 98.23, 108.65 |
Refinement procedure
| Resolution | 49.270 - 2.550 |
| R-factor | 0.20332 |
| Rwork | 0.202 |
| R-free | 0.21939 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.054 | 0.602 |
| Number of reflections | 93982 | |
| <I/σ(I)> | 11.9 | 1.4 |
| Completeness [%] | 95.9 | 91.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M sodium formate pH 7.0, 20 % (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
