3S7R

Crystal structure of a Heterogeneous nuclear ribonucleoprotein A/B (HNRPAB) from HOMO SAPIENS at 2.15 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97934,0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	43.150, 41.856, 55.146
Unit cell angles	90.00, 92.65, 90.00

Refinement procedure

Resolution	30.028 - 2.150
R-factor	0.1793
Rwork	0.177
R-free	0.22530
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.028	30.028	2.230
High resolution limit [Å]	2.150	4.610	2.150
Rmerge	0.086	0.034	0.571
Number of reflections	10785	2061	2151
<I/σ(I)>	7.05	18.6	1.6
Completeness [%]	97.3	97.2	96.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	277	40.00% polyethylene glycol 600, 0.1M CHES pH 9.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K