3S7G

Aglycosylated human igg1 fc fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-25
Detector	ADSC QUANTUM 315
Spacegroup name	P 1
Unit cell lengths	65.689, 65.773, 77.811
Unit cell angles	98.18, 100.50, 116.92

Refinement procedure

Resolution	45.000 - 3.130
R-factor	0.262
Rwork	0.259
R-free	0.32300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3DNK (CH2 AND CH3 DOMAINS USED SEPARATELY)
RMSD bond length	0.017
RMSD bond angle	1.633
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.130	3.130
Rmerge	0.061	0.347
Number of reflections	18883
<I/σ(I)>	12.6	2.8
Completeness [%]		97.1
Redundancy	1.9	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	10% PEG 1000, 20% PEG 6000, 0.1M MAGNESIUM CHLORIDE, 0.2M TRIS, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 295K