3S6W

Crystal structure of Tudor domain of human TDRD3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-08-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	41.930, 41.930, 61.478
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.310 - 1.780
R-factor	0.18397
Rwork	0.183
R-free	0.20618
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mhn
RMSD bond length	0.011
RMSD bond angle	1.138
Data reduction software	DENZO
Data scaling software	d*TREK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.310	1.920	1.840
High resolution limit [Å]	1.780	1.840	1.780
Number of reflections	5836
Completeness [%]	98.4	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	290	10% 2-propanol, 100mM Phosphate-Citrate, 0.2M Li2SO4, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K