3S4W
Structure of the FANCI-FANCD2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Detector technology | CCD |
| Collection date | 2009-12-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.800, 110.400, 350.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.858 - 3.408 |
| R-factor | 0.241 |
| Rwork | 0.240 |
| R-free | 0.27220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s51 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.881 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.092 | 0.591 |
| Number of reflections | 42995 | |
| <I/σ(I)> | 19.9 | 1.8 |
| Completeness [%] | 95.2 | 74 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 10 mM Hepes-Na, 40 mM potassium citrate, 10 mM DTT, 7 to 8 % (w/v) PEG 3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
