3S4R

Crystal structure of vimentin coil1A/1B fragment with a stabilizing mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	H 3 2
Unit cell lengths	65.329, 65.329, 377.339
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.520 - 2.452
R-factor	0.2929
Rwork	0.292
R-free	0.31470
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.217
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.450	6.650	2.450
Rmerge		0.026	0.420
Number of reflections	11209
<I/σ(I)>	14.5		1.7
Completeness [%]	93.7	99.1	55.9
Redundancy	10.9	10.2	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.1M Na cacodylate, MPD 20%, Mg acetate 0.45M, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.1M Na cacodylate, MPD 20%, Mg acetate 0.45M, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.1M Na cacodylate, MPD 20%, Mg acetate 0.45M, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 277K