3S4G
Low Resolution Structure of STNV complexed with RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1.488 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 313.146, 302.121, 183.385 |
Unit cell angles | 90.00, 93.82, 90.00 |
Refinement procedure
Resolution | 95.850 - 6.000 |
R-factor | 0.38 |
R-free | 0.43000 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 140.000 | 6.320 |
High resolution limit [Å] | 6.000 | 6.000 |
Rmerge | 0.128 | |
Number of reflections | 24647 | |
<I/σ(I)> | 11.8 | |
Completeness [%] | 99.0 | 97 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 0.4 % PEG 6000, pH 6.2-7.0, 1 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |