3S4C

Lactose phosphorylase in complex with sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9780
Spacegroup name	P 21 21 2
Unit cell lengths	85.450, 93.210, 106.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.820 - 2.400
R-factor	0.1826
Rwork	0.182
R-free	0.21410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.820	2.460
High resolution limit [Å]	2.400	2.400
Number of reflections	33519
Completeness [%]	98.9	89.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.6M ammonium sulfate, 0.1M Mes monohydrate pH6.5, 10% 1,4-dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 298K