3S4A
Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.690, 195.560, 103.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.790 - 1.990 |
| R-factor | 0.2025 |
| Rwork | 0.200 |
| R-free | 0.25490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.388 |
| Data reduction software | PROTEUM2 |
| Data scaling software | PROTEUM2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.790 | 2.050 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 118758 | |
| Completeness [%] | 98.8 | 90.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 4 M sodium formate, 20 mM GP, soaked with 5 M sodium formate, 20 mM cellobios , pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
