3S41
Glucokinase in complex with activator and glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.010, 82.200, 86.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.900 - 2.180 |
| R-factor | 0.1926 |
| Rwork | 0.190 |
| R-free | 0.24580 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3fgu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 82.200 |
| High resolution limit [Å] | 2.180 |
| Rmerge | 0.115 |
| Number of reflections | 22198 |
| Completeness [%] | 87.1 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, PH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
