3S3Z

Crystal Structure an Tandem Cyanovirin-N Dimer, CVN2L10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	200
Detector technology	CCD
Collection date	2008-05-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.99984
Spacegroup name	P 32 2 1
Unit cell lengths	47.990, 47.990, 79.318
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.810 - 1.750
R-factor	0.1895
Rwork	0.188
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ezm
RMSD bond length	0.011
RMSD bond angle	1.351
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	PHASER (1.3.3)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.560	79.318	1.840
High resolution limit [Å]	1.750	5.530	1.750
Rmerge	0.103	0.068	0.384
Total number of observations		1855	8422
Number of reflections	11158
<I/σ(I)>	12.7	7.6	1.7
Completeness [%]	100.0	98.5	100
Redundancy	5.3	4.5	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	298	20% PEG 3350, 0.2 M sodium fluoride, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K