3S3Y
Crystal Structure an Tandem Cyanovirin-N Dimer, CVN2L0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 47.865, 47.865, 78.684 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.930 - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ezm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.375 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.453 | 28.537 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.330 | 2.000 |
| Rmerge | 0.051 | 0.039 | 0.228 |
| Total number of observations | 1374 | 6020 | |
| Number of reflections | 7456 | ||
| <I/σ(I)> | 23.6 | 12.8 | 3.3 |
| Completeness [%] | 100.0 | 98.6 | 100 |
| Redundancy | 5.7 | 4.9 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.8M Na H2PO4, 0.8M K H2PO4, 100mM Sodium HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
