3S32
Crystal structure of Ash2L N-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2009-10-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9785 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 49.893, 49.893, 167.542 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.950 - 2.450 |
R-factor | 0.22734 |
Rwork | 0.225 |
R-free | 0.26342 |
Structure solution method | SAD |
RMSD bond length | 0.021 |
RMSD bond angle | 1.909 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.760 | 2.490 |
High resolution limit [Å] | 2.450 | 2.700 | 2.450 |
Number of reflections | 9437 | ||
<I/σ(I)> | 55.3 | ||
Completeness [%] | 99.6 | 100 | 100 |
Redundancy | 18.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2M MgAcetate and 17-20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |