3S32

Crystal structure of Ash2L N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	200
Detector technology	CCD
Collection date	2009-10-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9785
Spacegroup name	P 31 2 1
Unit cell lengths	49.893, 49.893, 167.542
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.950 - 2.450
R-factor	0.22734
Rwork	0.225
R-free	0.26342
Structure solution method	SAD
RMSD bond length	0.021
RMSD bond angle	1.909
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	2.760	2.490
High resolution limit [Å]	2.450	2.700	2.450
Number of reflections	9437
<I/σ(I)>	55.3
Completeness [%]	99.6	100	100
Redundancy	18.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.2M MgAcetate and 17-20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K