3S1R
RNA Polymerase II Initiation Complex with a 5-nt 3'-deoxy RNA soaked with GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-11 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.670, 221.320, 193.210 |
Unit cell angles | 90.00, 98.31, 90.00 |
Refinement procedure
Resolution | 29.970 - 3.200 |
R-factor | 0.1793 |
Rwork | 0.177 |
R-free | 0.22590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.300 |
Data scaling software | XSCALE |
Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.973 | 3.290 | |
High resolution limit [Å] | 3.200 | 14.320 | 3.200 |
Rmerge | 0.164 | 0.011 | 0.011 |
Number of reflections | 108840 | 1129 | 7741 |
<I/σ(I)> | 9.26 | 42.6 | 1.7 |
Completeness [%] | 99.4 | 88.1 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 293 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM dioxane, 9-11% PEG 6.000, 15mM DTT, vapor diffusion, temperature 293K |